Non-invasive assessment of the bioburden
of minced pork using
a hand-held fluorescence device
Christina Grimmler,
Tamara Kuhfuss,
Matthias Heiden,
Heinar Schmidt
Kapitel/Beitrag aus dem Buch: Längle, T et al. 2017. OCM 2017 – 3rd International Conference on Optical Characterization of Materials, March 22nd – 23rd, 2017, Karlsruhe, Germany : Conference Proceedings.
To speed-up monitoring of the hygienic status of meat,
fast and non-invasive measuring techniques are required. The
feasibility of a hand-held fluorescence device (freshdetect) was
investigated to quantify the bioburden of minced pork meat. In
total, 144 minced pork samples from 18 batches were stored at
2°C for up to 8 days. Fluorescence spectra, L*a*b* colour and total viable mesophilic plate counts (TVC) were measured in parallel during this period. Correlation of the fluorescence spectra
with log(TVC/g) using partial least squares regression yielded
cross-validated predictions with R²cv = 0.72 and a prediction error of RMSECV = 0.97 log(TVC/g). Cross-validated limits of
detection and quantification were determined as 1.03 and 3.38
log(TVC/g), respectively. This would allow for a quantification
well below the alarm threshold of 5.7-6.7 log(TVC/g). The spectra correlated also well with storage time (R²cv = 0.81), but less
strong with L*a*b* colour.